| Solvent Management | |
| Solvent Conditioning | Vacuum degas, three solvents plus purge solvent |
| Typical Operating Flow Rate Range | 0.050 to 5.000 mL/min in 0.001-mL/min increments |
| Programmable Flow Rate Range | .000 and 0.010 to 10.000 mL/min in 0.001-mL/min increments |
| Compressibility Compensation | Automatic and continuous |
| System Delay Volume | <400 µL, independent of backpressure, 1 mL/min |
| Plunger Seal Wash | Integral, active, programmable Gradient Profiles: Eleven (11) gradient curves [including linear, step (2 curves), concave (4 curves), and convex (4 curves)] |
| Gradient Profiles | Eleven (11) gradient curves [including linear, step (2 curves), concave (4 curves), and convex (4 curves)] |
| Dry Prime/Wet Prime | Automatic, front panel control |
| Flow Ramping | Time (0.01 to 30.00 min in 0.01-min increments) to reach maximum flow rate |
| Maximum Operating Pressure | 5000 psi (345 bar) at 0.010 to 3.000 mL/min; Programmable upper and lower limits |
| Composition Range | 0.0 to 100.0% in 0.1% increments |
| Composition Accuracy | ±0.5% absolute, independent of backpressure (Proportioning Valve Pair Test [degassed methanol: methanol/propylparaben, 2 mL/min, 257 nm]) |
| A Composition Precision |
<=0.15% RSD or<=0.02 min SD, whichever is greater, based on retention time. (Degassed 60% methanol:40% water Dial-a-Mix, 1 mL/min, six replicates, phenone mix, 257 nm) |
| Flow Precision | <=0.075% RSD or <=0.02 min SD, whichever is greater, based on retention time (N = 6) or volumetric measures (0.200 to 5.000 mL/min), isocratic premix |
| Flow Accuracy | <=1% or 10 µL/min, whichever is greater, (0.200 to 5.000 mL/ min), degassed methanol, at 600 psi backpressure |
|
Sample Management |
|
| umber of Sample Plates: | Total of four plates: – Microtiter plate – 96- or 384-well – Vial plate – 2.0-mL vial (48) – Tube plate – 0.5-mL microcentrifuge tube (48) or 1.5-mL microcentrifuge tube (24) – Open access plate – 2.0-mL vial (24 or 48) |
| Sample Temperature Control: | 4 to 40 °C, programmable in 1 °C increments |
| Maximum Sample Capacity | 1536 in four 384-well plates |
| Number of Sample Injections: | 1 to 99 injections per sample |
| Sample Delivery Precision: | <0.3% RSD, full loop 50 µL. (Default wash/purge conditions, degassed 60% methanol: 40% water Dial-a-Mix, 1 mL/min, six replicates, paraben mix, 257 nm) |
| Sample Volume Linearity | >0.999% correlation, 5 to 20 µL, partial fill in 50-µL loop, 3-µL pre- and post-sample air gaps (Default wash/purge conditions, degassed 60% methanol:40% water Dial-a-Mix, 1 mL/min, six replicates, paraben mix, 257 nm) |
| Needle Wash Solvents | Two: wash (strong solvent) and purge (sample- compatible) solvent A |
| Sample Carryover | <0.01% or <2.5 nL (default wash/purge volumes), whichever is greater. (Blank injection, 25 µL, 60% methanol:40% water, after six replicates, 25 µL propylparaben, 1 g/L, 257 nm) |
Waters 474 scanning fluorescence detector
| Condition | Specification |
| Monochromator | Holographic concave diffraction grating (for both excitation and emission) |
| Light source | 150 W Xenon lamp |
| Wavelength range | 200 to 700 nm (for excitation and emission). Can be set from 0 to 900 |
| Spectrum band width | Excitation: 18 nm Emission: 10, 18, or 40 nm (set on keypad) |
| Wavelength accuracy | ±2 nm |
| Wavelength repeatability | ±0.3 nm |
| Detector | Excitation: Photodiode Emission: Photomultiplier tube |
| Second order filter | Automatically inserted into light path when Ex > 400 Nm |
| Flow cell | 16 µL (standard) or 5 µL |
| Maximum flow cell pressure | 16 µL: 145 psi (10 bar) 5 µL: 500 psi (34.5 bar) |
| Wetted surfaces | Synthetic silica, fluororesin, PEEK, stainless steel SXS-316 |
| Sensitivity | S/N for the Raman peak of water is 320 or above, where Ex λ = 350 nm and time constant is 1.5 sec, 16 µL cell (Instruments shipped prior to June 1997, S/N for the Raman peak of water is 200 or above) |
| Attenuation range (Recorder output) | Ten steps, including 1, 2, 4, 8, 16, 32, 64, 128, 256, S |
| Gain range | 1, 10, 100, 1000 |
| Response | Fast, Standard, Slow, and digital filter |
| Signal processing | Digital processing by A/D and D/A converters (with surrounding temperature compensation circuits) |
| Outputs | • Recorder: 10 mV full-scale (can be inverted) • Integrator: 1 V full-scale • Marker output • Leak output |
| Inputs | • Marker input • Auto Zero input • Program reset |
| Program functions | Time program with following variables: • Wavelength change (excitation and emission) • Gain • Attenuation • Wavelength scan • Ex, Em Spectra |
| Wavelength scan functions | Manual and time program parameters: • Time • Interval • Amplitude • Memory location • Ex, Em Spectra |
| Spectrum storage | Ten locations for both emission and excitation spectra |
| Self-test diagnostics | • Memory (ROM and RAM) • DC power • Excitation energy decrease • Leak in cell • Lamp use time |
| Lamp use timer | Internal timer for accumulating the total hours on the Xenon lamp |
| Dimensions | Width: 11.81 inches (300 mm) Height: 5.90 inches (150 mm) Depth: 18.11 inches (460 mm) |
| Weight | 41.8 pounds (19 kg) |
| Power requirement | 115 VAC (90 to 132 V), 50/60 Hz 230 VAC (187 to 264 V), 50/60 Hz |
| Power consumption | 300 W maximum |
| Ambient temperature | 10 °C to 32 °C (50 °F to 90 °F) operation 10 °C to 60 °C (50 °F to 140 °F) storage |
| Relative humidity | 20 to 80%, non-condensing |
This refurbished Waters Alliance 2795 HPLC system comes with Waters 474 Scanning Fluorescence Detector.
We have other Waters HPLC detectors as option.
* Waters 486 Tunable Detector
* Waters 2487 Dual wavelength Detector
* Waters 996 PDA Detector
* Waters 2996 PDA Detector
This refurbished Waters Alliance 2795 HPLC system with Waters 474 scanning fluorescence detector is in great condition. It has been tested at SpectraLab Scientific.
We could sell them separately upon request.
A 90 day warranty is included. Multiple available.
We have special expertise on analytical equipment. We encourage you to contact us to speak with one of our expert and professional team members.
Thanks for looking and we look forward to hearing from you!
