This HP / Agilent 1200 Series HPLC system comes with the followings:
- G1322A Degasser
- G1311A Quaternary Pump
- G1329A Autosampler
- G1316A Thermostatted Column Compartment
- G1315B Diode Array Detector
- Solvent Tray
- PC with Suitable software and all essential cables required for full operation
This Agilent 1200 Series HPLC system is in a great shape. It has been tested at SpectraLab Scientific.
A 90 day warranty is included.
Performance Specifications
| G1311A Pump | |
|---|---|
| Type | Specification |
| Hydraulic system | Dual plunger in series pump with proprietary servo-controlled variable stroke drive, floating plungers and active inlet valve |
| Setable flow | 0.001 – 10 ml/min, in 0.001 ml/min increments |
| Flow precision | £ 0.07% RSD, or £ 0.02 min SD whatever is greater, based on retention time at constant room temperature |
| Flow accuracy | ± 1% or 10 µl/min whatever is greater |
| Pressure | Operating range 0 – 20 MPa (0 – 200 bar, 0 – 2950 psi) up to 10 ml/min |
| Pressure pulsation | pulsation < 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar) |
| Compressibility compensation | User-selectable, based on mobile phase compressibility |
| Recommended pH | 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel |
| Gradient formation | Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve Delay volume 800 – 1100 µl, dependent on back pressure |
| Composition Range | 0 – 95 % or 5 – 100 %, user selectable |
| Composition Precision | < 0.2 % RSD, at 0.2 and 1 ml/min |
| Control and data evaluation | Agilent control software (e.g. ChemStation, EZChrom, OL, etc.) |
| Communications | Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional |
| Safety and maintenance | Extensive diagnostics, error detection and display (through control module and Agilent Lab Monitor & Diagnostic Software), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas. |
| GLP features | Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with user-settable limits and feedback messages. Electronic records of maintenance and errors. |
| Housing | All materials recyclable |
| G1329A Autosampler | |
|---|---|
| Type | Specification |
| GLP features | Early maintenance feedback (EMF), electronic records of maintenance and errors |
| Communications | Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output |
| Safety features | Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display |
| Injection range | 0.1 – 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification required) |
| Replicate injections | 1 – 99 from one vial |
| Precision | Typically < 0.5 % RSD of peak areas from 5 – 100 µl, Typically < 1 % RSD of peak areas from 1 – 5 µl |
| Minimum sample volume | 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial |
| Carryover | Typically < 0.1 %, < 0.05 % with external needle cleaning |
| Sample viscosity range | 0.2 – 50 cp |
| Replicate injections per vial | 1 – 99 |
| Sample capacity | 100 × 2-ml vials in 1 tray 40 × 2-ml vials in ½ tray 15 × 6-ml vials in ½ tray (Agilent vials only) |
| Injection cycle time | Typically 50 s depending on draw speed and injection volume |
| G1316A TCC | |
|---|---|
| Type | Specification |
| Temperature range | 10 degrees below ambient to 80 °C up to 80 °C: flow rates up to 5 ml/min |
| Temperature stability | ± 0.15 °C |
| Temperature accuracy | ± 0.8 °C ± 0.5 °C (with calibration) |
| Column capacity | Three 30 cm |
| Warm-up/cool-down time | 5 minutes from ambient to 40 °C 10 minutes from 40 – 20 °C |
| Dead volume | 3 µl left heat exchanger 6 µl right heat exchanger |
| Communications | Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN via other 1200 series module |
| Safety and maintenance | Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas. |
| GLP features | Column-identification module for GLP documentation of column type |
| Housing | All materials recyclable |
| G1315B DAD | |
|---|---|
|
Wavelength range |
190-950 nm |
|
Light source |
Deuterium and tungsten lamps |
|
Detection type |
1024 – element photodiode array |
|
Short term noise (ASTM) single & mult-wavelength |
± 1° x 10-5 AU at 254 and 750 nm |
|
Drift |
2° x 10-3 AU / hr at 254 nm |
|
Linear absorbance range |
> 2 AU (upper limit) |
|
Wavelength accuracy |
± 1 nm |
|
Wavelength bunching |
1 – 400 nm |
|
Diode width |
< 1 nm |
|
Flow cells |
Standard: 13µl volume 10 mm cell path length and 120 bar (1760 psi) pressure maximum |
|
Analog outputs |
Recorder / integrator: 10 mV or 1 V, output range |
|
Control and data evaluation |
Agilent Chemstation for LC |
|
Slit width |
1, 2, 4, 8, 16 nm |
|
Communication types |
CAN, GBIP, RS-232C, APG, Remote: ready, start, stop and shut down |
|
Wavelength range |
190-950 nm |
|
Light source |
Deuterium and tungsten lamps |
|
Detection type |
1024 – element photodiode array |
|
Short term noise (ASTM) single & mult-wavelength |
± 1° x 10-5 AU at 254 and 750 nm |
|
Drift |
2° x 10-3 AU / hr at 254 nm |
|
Linear absorbance range |
> 2 AU (upper limit) |
