This system includes:
- (1) AB Sciex TripleTOF 5600 Mass Spectrometer
- (1) Eksigent nanoLC as-2 Autosampler
- (1) Eksigent nanoLC ultra 2D Pump
- (1) Eksigent eks-PS-250 Power Supply
- (1) PC Data System
- (1) SogeVac SV65 BI FC Rotary Vane Pump
AB Sciex TripleTOF 5600:
- Enhanced Selectivity with Differential Ion Mobility
- Get more out of every sample with an additional dimension of separation with SelexION Differential Ion Mobility Technology.
- Robust Spray with the NanoSpray III Source
- Efficient ion production while limiting contamination for maximized robustness, reliability and uptime.
- Comprehensive Coverage with Data-Independent SWATH Acquisition
- Variable window capabilities allow for a large number of Q1 isolation windows at user controlled widths, up to 200 per cycle to achieve better specificity, even in complex samples.
- Maximize Flexibility with Front End Separations
- Compatible with advanced front-end separation strategies including high-flow, microflow and nanoflow, and capillary electrophoresis spray ionization (CESI).
Features:
| Mass Range TOF | 40 kDa |
| Mass Selection Range Q1 | 5 – 1250 m/z |
| TOF-MS Mass Resolution and Acquisition Speed | ≥35,000 (FWHM) at 956 m/z, using 50 ms accumulation time |
| TOF-MS/MS Mass Resolution and Acquisition Speed | ≥25,000 (FWHM) at 195 m/z, and ≥18,000 (FWHM) at 86 m/z, using 50 ms accumulation time |
| Detector Type | 4-channel MCP detector with TDC signal processing for extended dynamic range |
| Flexible Data Acquisition Workflows | TOF-MS Information dependent acquisition (IDA) MS/MS Data Independent SWATH Acquisition with fixed or variable Q1 isolation windows Targeted MRMHR workflow |
| Ionization Sources | |
| Compatible with advanced front-end separation strategies including high-flow, microflow and nanoflow chromatography, capillary electrophoresis spray ionization (CESI), or using SelexION differential ion mobility | |
| Variable Window SWATH Acquisition | Data-Independent SWATH Acquisition with variable window capabilities, allows for a large number of Q1 isolation windows at user controlled widths, up to 200 per cycle to achieve better specificity, even in complex samples. |
| Data File Portability | Condensed data file sizes simplify and streamline data storage and portability for sharing with colleagues, without losses in data quality, completeness or security |
Eksigent nanoLC as-2 Autosampler:
- Flexible and easy-to-use
- Employs a 2.4 ul volume needle for injection of small volumes without sample losses
- The needle is coated with a proprietary inert coating on both the inside and outside to prevent sample losses due to adsorption.
- The sample compartment is completely enclosed to ensure temperature control down to 4°C.
- Capacity is two microtiter plates or 96 vials. Using two different wash positions, the needle is washed inside and outside to reduce carry-over.
- The 6-port injection valve has port-to-port volumes of <25 nl, and is rated to 10,000 psi.
- A proprietary coating on the valve stator and rotor assures excellent valve reliability and reduces sample adsorption.
Eksigent nanoLC ultra 2D Pump:
- NanoLC-Ultra 2D systems include all of the features of the 1D, plus the added capability of automated 2-dimensional chromatography.
- The NanoLC-Ultra 2D (two gradients) system and NanoLC-Ultra 2Dplus system (two gradients and one isocratic loading pump) are designed for advanced applications such as on-line 2D LC or high throughput set-ups using two nanoLC columns.
- All NanoLC-Ultra 2D and 2Dplus systems are available with one or two built-in 10-port valves.
- NanoLC-Ultra 2D and 2Dplus system can be configured to run in either 2-dimensional separation mode or as two parallel, completely independent binary gradient HPLC systems
Eksigent eks-PS-250 Power Supply:
- 100-127/200-240V, 4/2A, 50/60Hz
- DC24V/DC5V, 8.5A/2.5A
SogeVac SV65 BI FC Rotary Vane Pump:
- Pumping speed (50/60 Hz): 22.7 / 27.7 cfm
- Ultimate pressure: < 1.1 Torr
- Mains connection: 200-240V, 1-ph, 50/60 Hz
- Inlet connection: KF-40
