Q Exactive HF-X Quadrupole-Orbitrap Mass Spectrometer
The Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap mass spectrometer combines a high-resolution, accurate-mass (HRAM) ultra-high-field Orbitrap mass analyzer, high capacity transfer tube with electrodynamic ion funnel and high performance quadrupole precursor ion selection to deliver unsurpassed speed and first class sensitivity.
The Q Exactive HF-X MS takes identification and quantitation to an unmatched level, making it possible to identify, quantify, and confirm in a single analysis with a single instrument.
The Q Exactive HF-X MS provides best-in-class performance in peptides, proteins, and small molecule applications. Accelerated Higher Energy Collisional Dissociation (aHCD) allows for MS/MS acquisition up to 40Hz. Advanced Quadrupole Technology (AQT) optimizes precursor ion selection and transmission, improving quantitation of low-abundant ions in the most complex matrices. Sophisticated Parallel Reaction Monitoring and Data-Independent Acquisition (DIA) methods provide reproducible quantitation results while delivering complete qualitative confidence. 240,000 maximum resolving power at m/z 200 (FWHM), ensures more confident structural confirmation in biopharma and omics applications. The new BioPharma option adds superior capabilities when it comes to the analysis of native proteins or protein complexes and their subunits (top/middle-down), and peptide mapping characterization.
Features:
• Scan speed up to 40 Hz with ultra-high-field Thermo Scientific Orbitrap mass analyzer technology
• Resolution of up to 240,000 (FWHM) at m/z 200
• High Capacity Transfer Tube (HCTT) and electrodynamic ion funnel for improved sensitivity
• Advanced Quadrupole Technology (AQT) for superior precursor isolation
• Advanced Active Beam Guide (AABG) for greater sensitivity and maximum robustness
• New Data Dependent Acquisition (DDA) strategy for bottom-up and top-down proteomics analysis
• Dynamic retention time adjustment in PRM for superior targeted quantitation
• Accelerated Higher-Energy Collisional Dissociation (aHCD) capabilities
• BioPharma option for enhanced analysis of intact proteins, protein-protein complexes, mAb subunits, and intact mAb. Includes Intact Protein Mode and High Mass Range (HMR) mode.
EASY-nLC 1000
The Thermo Scientific EASY-nLC 1000 provides effortless split-free, nano-flow UHPLC performance up to 1000 bar with minimal investment. Dual in-line flow sensors before mixing gives uncompromising gradient precision.
Features:
Advanced Flow Control
• Intelligent Flow Control (IFC) during loading and equilibration provides robust performance and protects against blocked columns
• Advanced Flow Control (AFC) during gradients provides outstanding reproducibility, sample to sample and over time
Intelligent Maintenance
• Automated, built-in maintenance steps
• Remote access via internet for system interrogation
• Event log for troubleshooting
• Automated leak test indicates actual position of leak
• System back pressure test quickly detects flow-line blockage
Integrated Design with Built-in PC
• Intuitive control from touch screen interface
• Structured layout for easy component exchange
• Pulsation-free pumps minimize need for replacement of seals, valves and fittings
• Optimized flow paths are utilised by software
• Easy maintenance, access and service
Stable, Pulsation-free Gradients from 100 nL/min
• Factory-configured flow paths minimize swept volume
• Maximum chromatographic accuracy with:
– Split-free gradient mixing
– Step-controlled nano-flow syringe pumps
– Flow sensor control of mobile phase delivery
System Includes:
- Q Exactive HF-X Quadrupole-Orbitrap Mass Spectrometer
- EASY-nLC 1000
- Chemyx Fusion 100T2 Syringe Pump
- Agilent MS120 Single Stage Rotary Vane Pump
- PC with xCalibur software
