This HP/ Agilent 1200 Series Nano HPLC System comes with the followings:
- G1379B Degasser with Tray
- G2226A Nano Pump
- G1377A Micro WPS Autosampler
- G1316A TCC
- G1315B Diode Array Detector
- PC with data and all essential cables
This system is in great condition. It has been tested at SpectraLab Scientific. A 90 day warranty is included.
We encourage you to contact us to speak with one of our expert and professional team members. Thanks for looking and we look forward to hearing from you!
Performance Specifications:
| G2226A Nano Pump | |
|---|---|
| Type | Specification |
| Hydraulic system | Two dual piston in series, with proprietary servo-controlled variable stroke drive, floating piston, active inlet valve, solvent selection valve and electronic flow control for flow rates from 0.1 to 1 µl/min |
| Settable column flow range | 0.01 – 4 µl/min 1 – 2500 µl/min (with the electronic flow control bypassed) |
| Recommended column flow range | 0.1 – 1 µl/min 200 – 2500 µl/min (with the electronic flow sensor bypassed) |
| Optimum composition range | 1 to 99% or 5 µl/min per channel (primary flow), whatever is greater |
| Composition precision | < 0.2 % SD, at 500 nl/min (default settings), Minimum primary flow/pump channel is 5 µl/min |
| Delay volume | Typically 300 nl from the electronic flow control to the pump outlet for flow rates up to 4 µl/min. For flow rates up to 4 µl/min and electronic flow control active: primary flow path 180 – 480 µl; system pressure dependent (default settings; calculated volume)Typically 180 to 480 µl (system pressure dependent) for flow rates up to 2.5 ml/min. (default settings; calculated volume) |
| Pressure range | 20 to 400 bar (5880 psi) system pressure |
| Compressibility compensation | User-selectable, based on mobile phase compressibility |
| Recommended pH range | 1.0 – 8.5, solvents with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries. |
| Control and data evaluation | Agilent ChemStation for LC |
| Analog output Communications | For pressure monitoring, 2 mV/bar, one output Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional |
| Safety and maintenance | Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas. |
| GLP features | Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with user-settable limits and feedback messages. Electronic records of maintenance and errors. |
| Housing | All materials recyclable. |
| G1379B Degasser | |
|---|---|
| Type | Specification |
| Maximum flow | 5 ml/min per channel |
| Number of channels | 4 |
| Internal volume | Typically 1 ml per channel |
| pH range | 1-14 |
| RS232 output | For diagnosis purpose |
| Material in contact with Solvents | PTFE, FEP, PEEK |
| G1377A Micro WPS | |
|---|---|
| Type | Specification |
| GLP features | Early maintenance feedback (EMF), electronic records of maintenance and errors |
| Communications | Communications Controller-area network (CAN). RS232C, APG-remote standard, optional four external contact closures and BCD vial number output |
| Safety features | Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display |
| Injection range | 0.01– 8 µl in 0.01 µl increments with the small loop capillary 0.01–40 µl in 0.01 µl increments with the extended loop capillary |
| Precision | Typically < 0.5% RSD of peak areas from 5 – 40 µl, Typically < 1% RSD from 1 – 5 µl Typically < 3% RSD from 0.2 – 1 µl |
| Pressure range | 400 bar (5880 psi) |
| Sample viscosity range | 0.2 – 5 cp |
| Sample capacity | 2 × well-plates (MTP) + 10 × 2 ml vials 108 x 2-mL vials in 2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6-mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials 54 Eppendorf tubes (0.5/1.5/2.0 mL) in 2 x 27 Eppendorf tube plate |
| Injection cycle time | Typically < 30 s using following standard conditions: Default draw speed: 4 µl/min Default eject speed: 10 µl/min Injection volume: 0.1 µl |
| Carry-over | Typically < 0.05% using the following conditions: Column: 150 x 0.5 mm Hypersil ODS, 3 µm Mobile phase: Water/Acetonitrile = 85/15 Column Flow rate: 13 µl/min Injection volume: 1 µl caffeine (=25 ng caffeine), 1 µl water to test carry over Outside wash of needle before injection: 20 sec with water using flush port |
| G1316A TCC | |
|---|---|
| Type | Specification |
| Temperature range | 10 degrees below ambient to 80 °C up to 80 °C: flow rates up to 5 ml/min |
| Temperature stability | ± 0.15 °C |
| Temperature accuracy | ± 0.8 °C ± 0.5 °C (with calibration) |
| Column capacity | Three 30 cm |
| Warm-up/cool-down time | 5 minutes from ambient to 40 °C 10 minutes from 40 – 20 °C |
| Dead volume | 3 µl left heat exchanger 6 µl right heat exchanger |
| Communications | Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN via other 1200 series module |
| Safety and maintenance | Extensive diagnostics, error detection and display (through control module and Agilent Chem Station), leak detection, safe leak handling, leak output signal for shut down of pumping system. Low voltages in major maintenance areas. |
| GLP features | Column-identification module for GLP documentation of column type |
| Housing | All materials recyclable |
| G1315B Diode Array Detector | |
|---|---|
| Wavelength range | 190-950 nm |
| Light source | Deuterium and tungsten lamps |
| Detection type | 1024 – element photo diode array |
| Short term noise (ASTM) single & mult-wavelength | ± 1° x 10-5 AU at 254 and 750 nm |
| Drift | 2° x 10-3 AU / hr at 254 nm |
| Linear absorbance range | > 2 AU (upper limit) |
| Wavelength accuracy | ± 1 nm |
| Wavelength bunching | 1 – 400 nm |
| Diode width | < 1 nm |
| Flow cells | Standard: 13µl volume 10 mm cell path length and 120 bar (1760 psi) pressure maximum Semi-Micro: 5µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum Micro: 2µl volume, 3 mm cell path length and 120 bar (1760 psi) pressure maximum High Pressure: 1.7µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure max. 80 nano: 0.08µl volume, 100 mm cell path length and 50 bar (725 psi) pressure max. 500 nano: 0.5µl volume, 10 mm cell path length and 50 bar (725 psi) pressure max. |
| Analog outputs | Recorder / integrator: 10 mV or 1 V, output range 0.001 – 2 AU, two outputs |
| Control and data evaluation | Agilent Chem station for LC |
| Slit width | 1, 2, 4, 8, 16 nm |
| Communication types | CAN, GBIP, RS-232C, APG, Remote: ready, start, stop and shut down |
| Wavelength range | 190-950 nm |
| Light source | Deuterium and tungsten lamps |
| Detection type | 1024 – element photo diode array |
| Short term noise (ASTM) single & mult-wavelength | ± 1° x 10-5 AU at 254 and 750 nm |
| Drift | 2° x 10-3 AU / hr at 254 nm |
| Linear absorbance range | > 2 AU (upper limit) |
