The Agilent QTOF 6540 is an accurate mass MSMS instrument based upon quadrupole and time-of-flight technology. The instrument accommodates several different solution-based atmospheric pressure ionization sources including electrospray (ESI), chemical ionization (APCI), and JetStream ESI. The instrument can acquire data in positive or negative ion mode, MS, or MS/MS mode. Agilent MassHunter software is used for data acquisition and data processing. The software package includes modules for determining elemental composition, molecular feature extractor, deconvolution, et cetera.
Working Mode of Agilent 6540 Q-TOF Mass Spectrometer
TOF Mode
The Quadrupole is under the situation of Total Transmission Ion (TTI), which means all ions can pass the quadrupole. The collision cell does not fragment the ions and works as ion transmission devices. The TOF mass analyzer is in the scan mode and can directly detect the ions. The mass spectrum obtained from the TOF mode is equivalent to the one from the single TOF mass analyzer.
Auto MS/MS Mode
In this mode, the instrument can automatically perform secondary mass spectrometry on qualified ions according to the conditions set by users. When a particular ion or a set of ions meet the set conditions, the quadrupole is in the selected ion monitoring (SIM), and the collision cell can fragment ions. Then the fragment ions can be scanned by TOF and get a mass spectrum. The auto MS/MS mode is commonly used for unknown compounds identification and structure analysis.
Targeted MS/MS Mode
In this mode, only the ions targeted by users can get mass spectrum. Similar to the auto MS/MS mode, the quadrupole is in SIM, and the collision cell can fragment ions. Then the fragment ions can be scanned by TOF and get a mass spectrum. This mode is useful for the quantitative analysis, identification of known compounds, and structural elucidation.
Instrument characteristics include:
- LC pumps can handle up to 8000 psi
- MS and MS/MS on the fly
- Mass Range of up to 10,000 Daltons (singly-charged)
- Mass Accuracy of better than 3 PPM
- Resolution (FWHM) 45,000 for mass 1000
- Rapid switching between positive and negative ion modes
Tested & refurbished. Front end Agilent 1260 HPLC system includes:
- G1312B SL Binary Pump
- G1329B Autosampler
- G1316B Thermostatted Column Compartment SL
- G1322A Vacuum Degasser
- G1330B Autosampler Thermostat
Comes with a 90-day warranty.
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