Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer 2018 model. A rough pump and PC with data is included. We can add any suitable UHPLC/HPLC to this MS system.
| Performance Characteristics | |
| Resolution | 240,000 at m/z 200 |
| Mass range | 50 to 6,000 m/z |
| Scan rate* | Up to 40 Hz at resolution setting of 7,500 at m/z 200 |
| Mass accuracy* | Internal: < 1 ppm RMS
External: < 3 ppm RMS |
| Sensitivity | MS/MS: 100 fg reserpine on column S/N 150:1
SIM: 50 fg reserpine on column S/N 150:1 |
| Dynamic range | > 5,000:1 |
| Polarity switching | One full cycle in < 1 sec (one full positive mode scan and one full negative mode scan at a resolution setting of 60,000) |
| Multiplexity | Up to 10 precursors/scan |
| Analog inputs | One (1) analog input (0–1 V)
One (1) analog (0–10 V) |
Experience the cutting-edge capabilities of the Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap MS System. This advanced mass spectrometer combines innovative technologies to deliver unmatched speed, sensitivity, and precision in identification and quantitation.
Unparalleled Performance: With its high-resolution, accurate-mass (HRAM) ultra-high-field Orbitrap mass analyzer, the Q Exactive HF-X MS ensures exceptional results across a wide range of applications. Achieve maximum resolving power of up to 240,000 (FWHM) at m/z 200, allowing for confident structural confirmation in biopharmaceutical and omics studies. The system offers superior performance in peptides, proteins, and small molecule analysis.
Enhanced Speed and Sensitivity: Experience the power of accelerated Higher-Energy Collisional Dissociation (aHCD) capabilities, enabling MS/MS acquisition at an impressive speed of up to 40 Hz. The advanced quadrupole technology (AQT) optimizes precursor ion selection and transmission, enhancing quantitation of low-abundant ions even in the most complex matrices. The high capacity transfer tube (HCTT) and electrodynamic ion funnel improve sensitivity, ensuring efficient ion flux and desolvation.
Advanced Acquisition Strategies: The Q Exactive HF-X MS features a new Data Dependent Acquisition (DDA) strategy for both bottom-up and top-down proteomics analysis. Benefit from dynamic retention time adjustment in PRM, enabling superior targeted quantitation. Additionally, sophisticated Parallel Reaction Monitoring and Data-Independent Acquisition (DIA) methods provide reproducible quantitation results and qualitative confidence.
BioPharma Option: Take your analysis to the next level with the BioPharma option. This enhanced feature enables comprehensive analysis of intact proteins, protein-protein complexes, mAb subunits, and intact mAb. It includes Intact Protein Mode and High Mass Range (HMR) mode, catering to the specific needs of native protein or protein complex characterization, as well as peptide mapping.
Cutting-Edge Hardware Specification: The Q Exactive HF-X MS is equipped with top-of-the-line hardware components for optimal performance and reliability. The Thermo Scientific Ion Max API Source includes an H-ESI II probe with dual desolvation zone technology, offering enhanced sensitivity and ruggedness. The High Capacity ion Transfer Tube (HCTT) and Electrodynamic Ion Funnel (EDIF) ensure improved ion flux, efficient ion capture, and reduced losses. The Advanced Quadrupole Technology (AQT) with a segmented Thermo Scientific HyperQuad mass filter provides superior ion transmission and isolation window shape.
Unmatched Data Acquisition: Experience ultra-fast real-time data acquisition and instrument control with the Q Exactive HF-X MS. The fully automated system calibration via instrument control software ensures accuracy and reliability. The Automatic Gain Control (AGC) support optimizes signal intensity for precise measurements.
Discover the Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap MS System and unlock the true potential of your analytical research. Achieve exceptional results with unrivaled speed, sensitivity, and confidence in your analyses.
