The Waters Sample Manager Fixed-Loop (SM-FL) offers lowest dispersion performance and is suitable for running 1.0 mm columns. The ACQUITY UPLC I-Class System is available with a choice of column management options.
| Type | Specifications |
|---|---|
| Total system bandspread, 5σ | 4 to 7uL Test conditions: • Mobile phase: 90:10 water/acetonitrile • Flow rate: 0.1 to 1.0 mL/min in 0.1 mL/min increments • Weak wash: 90:10 water/acetonitrile • Strong wash: 90:10 water/acetonitrile • Sample: 0.01 mg/mL caffeine (1-ul injection volume); 0.030 mg/mL caffeine (0.5-ul injection volume); 0.050 mg/mL caffeine (0.2-ul injection volume) • Sample loop: 10 uL • Injection mode: PLUNO • Column compartment: CH-A |
| Total system dwell volume | <95uL, default configuration |
| Integrated leak management | Leak sensors, as standard, and safe leak handling. Drip trays direct all leaks to the front of the instrument, and then into waste line. |
| Operating flow rate range | 0.010 to 2.000 mL/min, in 0.001 mL increments |
| Maximum operating pressure | • 124,106 kPa (1241 bar, 18,000 psi) up to 1mL/min • 82,737 kPa (827 bar, 12,000 psi) up to 2mL/min |
| pH range | pH 2 to 12 |
| Gradient mixers | Binary solvent manager: •50 uL mixer/filter • 100 uL mixer/filter • 380 uL mixer/filter |
| Unattended operation | Leak sensors, full 96-hour diagnostic data display through ACQUITY UPLC console software |
| Injection cycle time | <15s inject to inject, with load ahead enabledTest conditions: • System: BSM, SM-FL, ACQUITY PDA, CH-A • Solvent A: water/acetonitrile, 90:10 • Solvent B: 100% acetonitrile • Weak Wash: water/acetonitrile, 90:10 • Strong Wash: water/acetonitrile, 90:10 • Column: ACQUITY UPLC BEH C18 1.7 μm, 2.1 × 50 mm • Sample: caffeine at 0.04 mg/mL in water/acetonitrile, 90:10 • Mobile Phase: 100% solvent A • Isocratic chromatography •Flow rate: 0.4mL/min • Injection volume: 1uL • Load ahead mode: enabled • Run time: 2.0 min |
